This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with human CD13. Clone R3-63 has been reported to block mouse APN enzyme activity. We recommend the use of SM1856A for this purpose.
Purification
Affinity chromatography on Protein G
Immunogen
Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C
Storage Comment
Prior and Following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150