MRP8 / MRP14 antibody

Details for Product No. ABIN119773, Supplier: Log in to see
Antigen
Reactivity
Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit, Rat (Rattus)
32
9
9
6
6
6
6
6
6
6
2
2
2
Host
Mouse
16
13
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
16
13
10
7
7
4
4
Options
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Immunogen Human Monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone MAC387
Isotype IgG1
Specificity This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 12 kDa beta chain expressed by Granulocytes, Monocytes and by tissue Macrophages. Variable results have been reported for staining brain macrophages and microglia.
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Porcine, Canine, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Feline, Cynomologus monkey, Rhesus monkey and Goat.
Purification Affinity Chromatography on Protein G
Background Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).Synonyms: MRP8/14, S100A8, S100A8/A9, S100A9
Research Area Cell Cycle
Application Notes Immunohistochemistry on Frozen sections (1/100-1/200). Immunohistochemistry on Paraffin Embedded Sections (1/100-1/200): requires protein digestion pre-treatment e.g. trypsin, 0.1 % for 10 minutes or antigen retrieval using heat treatment prior to staining, see also Ref.2. Recommended Positive Control Tissue: Human Spleen. Flow Cytometry (1/50-1/100): membrane permeabilisation required, see also Ref.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-MRP8 / MRP14 antibody (ABIN119773) Macrophages stained with ABIN119773 using enhanced DAB in experimental allergic marmo...
Background publications Obert, Hoover: "Early pathogenesis of transmucosal feline immunodeficiency virus infection." in: Journal of virology, Vol. 76, Issue 12, pp. 6311-22, 2002 (PubMed).

Bagavant, Sharp, Kurth, Tung: "Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)." in: The American journal of pathology, Vol. 160, Issue 1, pp. 141-9, 2002 (PubMed).

Gutierrez, Forster, McConnell, Cassidy, Pollock, Bryson: "The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques." in: Veterinary immunology and immunopathology, Vol. 71, Issue 3-4, pp. 321-34, 1999 (PubMed).

Christgau, Caffesse, Newland, Schmalz, DSouza: "Characterization of immunocompetent cells in the diseased canine periodontium." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 46, Issue 12, pp. 1443-54, 1999 (PubMed).

Pérez, Mozos, Martín, Day: "Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma." in: Journal of comparative pathology, Vol. 121, Issue 4, pp. 385-97, 1999 (PubMed).

Malik, Francis, Holt, Gunn, Thomas, Shepherd, Chamberlain, Newman, Cumberland, Crossman: "Apoptosis and cell proliferation after porcine coronary angioplasty." in: Circulation, Vol. 98, Issue 16, pp. 1657-65, 1998 (PubMed).

Brandtzaeg, Dale, Gabrielsen: "The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function." in: Histopathology, Vol. 21, Issue 2, pp. 191-6, 1992 (PubMed).

Ramsay, Giddings, Baskerville, Cranage: "Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies." in: The Journal of pathology, Vol. 164, Issue 4, pp. 321-8, 1991 (PubMed).

Brandtzaeg, Jones, Flavell, Fagerhol: "Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 963-70, 1989 (PubMed).

Flavell, Jones, Wright: "Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 35, Issue 11, pp. 1217-26, 1987 (PubMed).