p53 antibody (AA 181-190)
Quick Overview for p53 antibody (AA 181-190) (ABIN120058)
Target
See all p53 (TP53) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 181-190
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Specificity
- This antibody recognises the p53 tumour suppressor protein, binding to both wild type and mutant forms. DO-11 recognises an epitope within the central region of p53 between amino acids 181 - 190.
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Purification
- Affinity chromatography on Protein G
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Immunogen
- Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
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Isotype
- IgG1
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 Reactivity: Human WB, IF, IHC (p), IP, ICC, ChIP Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 Reactivity: Human WB, ELISA, IF, IHC (p), IP Host: Mouse Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 Reactivity: Human WB, ELISA, IHC (p), PLA, RNAi Host: Mouse Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer15) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 Reactivity: Human, Mouse, Rat, Goat WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 293-322) antibodyTP53 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB7777 unconjugated
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Application Notes
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Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000, This product does require antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Colon or breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- p53 (TP53)
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Background
- Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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Gene ID
- 7157
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NCBI Accession
- NP_000537
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UniProt
- P04637
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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