This antibody recognises the CD178 cell surface antigen, a 40 kDa glycoprotein also known as Fas ligand (CD95L). Clone 10F2 is reported to block the activity of Fas ligand.
Purification
Affinity chromatography on Protein G
Endotoxin Level
Low Endotoxin
Immunogen
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Flow Cytometry: 1/25 - 1/50, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. ELISA: This antibody may be used in combination with clone 14C2 (ABIN120319) in ELISA assays. Immunoprecipitation. Functional assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
FASL
(Fas Ligand (TNF Superfamily, Member 6) (FASL))
CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6