LAG3 antibody
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- Target See all LAG3 Antibodies
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This LAG3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Functional Studies (Func)
- Specificity
- This antibody recognises lymphocyte activation gene-3 (LAG-3) that is also referred to as CD223. Clone C9B7W recognises an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II.
- Purification
- Affinity chromatography on Protein G
- Endotoxin Level
- Low Endotoxin
- Immunogen
- Murine CD223 Ig fusion protein. Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
- Clone
- C9B7W
- Isotype
- IgG1
- Top Product
- Discover our top product LAG3 Primary Antibody
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anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 103-132) antibody
LAG3 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB21775 unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) antibodyLAG3 Reactivity: Human WB, ELISA, IHC, FACS, ICC, Neut Host: Mouse Monoclonal 3A4A10 unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 103-132) antibodyLAG3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 29-450) antibodyLAG3 Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 8B12E10 unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (N-Term) antibodyLAG3 Reactivity: Human WB, ELISA, IHC, IP, FACS, ICC Host: Mouse Monoclonal L4-PL33 unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 1-360) antibodyLAG3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 244-487) antibodyLAG3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 29-450) antibodyLAG3 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 209-451) antibodyLAG3 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Lymphocyte-Activation Gene 3 (LAG3) (AA 201-300) antibodyLAG3 Reactivity: Human, Mouse WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01EU/ug - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody at -20 °C. Ship at 2 - 8 °C.
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- Target
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Alternative Name
- CD223 / LAG3 (LAG3 Products)
- Synonyms
- CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody
- Background
- CD223 is a 70kD activation-induced cell surface molecule. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.Synonyms: FDC, LAG-3, Lymphocyte activation gene 3 protein
- Gene ID
- 16768
- NCBI Accession
- NP_032505
- UniProt
- Q61790
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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