Transferrin Receptor (p90, CD71) (TFRC) antibody

Antigen: Transferrin Receptor (p90, CD71) (TFRC)

Synonyms: TFR, CD71, TFR1, TRFR, TR, p90, Trfr, Mtvr1, Mtvr-1, AI195355, AI426448, AU015758, 2610028K12Rik, E430033M20Rik, TFRC, tfr, cd71, tfr1, trfr, DKFZp469M0811, LOC100219280

Clonality: Monoclonal (OX-26)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunoelectron Microscopy (IEM), Flow Cytometry (FACS)

Catalog no.: ABIN120509

Additional Information

Alternative name: CD71 / TFRC

UniProt: Q99376

Immunogen: PHA activated rat lymphocytes. Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line

Cross-Reactivity: Rat (Rattus)

Isotype: IgG2a

Clone: OX-26

Description: CD71 is a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimers, Parkinsons, Huntingtons and Multiple Sclerosis. Alternate Names: Transferrin Receptor Protein 1, TFRC, TfR1, TfR, TR, Trfr, T9, p90.

Application Details

Application Notes: Flow Cytometry: Use 10ul of 1/50-1/200 diluted antibody to label 10e6 cells in 100 ul. Western Blot: This antibody detects a band of approximately 95kDa in ConA cell lysates under Reducing conditions (MWT 195kDA without reduction). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Clone MRC OX-26 is reported as suitable for use in Electron Microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Jefferies, Brandon, Williams et al.: "Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor." in: Immunology, Vol. 54, Issue 2, pp. 333-41, 1985 (PubMed).

Jefferies, Brandon, Hunt et al.: "Transferrin receptor on endothelium of brain capillaries." in: Nature, Vol. 312, Issue 5990, pp. 162-3, 1984 (PubMed).