CD8 antibody

Catalog No. ABIN120618

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The antibody recognizes CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Leucocytes of a patient suffering from LGL Type Leukaemia

Clone: MEM-87

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation (Excellent for immunoisolation of CD8 + T cells). Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. CD8 is a disulfide-linked dimer and exists as a CD8a homodimer or CD8a/b heterodimer (each monomer approx. 32-34 kDa).Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

UniProt: P01732