Cytokeratin 7 antibody

Catalog No. ABIN120627

The Mouse Monoclonal anti-Cytokeratin 7 antibody (Clone C-46) (ABIN120627) specifically detects Cytokeratin 7 in WB, IF, IHC (p), IP and IHC (fro). The antibody is reactive with Human and Cow samples.

Quick Overview for Cytokeratin 7 antibody (ABIN120627)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C-46

Product Details anti-Cytokeratin 7 Antibody

Specificity: The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).

Cross-Reactivity (Details): Species reactivity (tested):Human, Porcine, Bovine.
Also reacts to: KRT17

Characteristics: Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7

Purification: Protein A-affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7

Background: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

Gene ID: 3855

UniProt: P08729