Mouse anti-Human Ig (Light Chain), (Chain kappa) Antibody

Details for Product No. ABIN120648, Supplier: Log in to see
Antigen
Epitope
Light Chain, Chain kappa
540
393
289
66
25
16
13
10
7
6
5
3
2
2
1
Reactivity
Human
684
213
74
30
19
15
10
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
405
391
158
78
21
7
4
1
1
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen Crude thymus membrane fraction
Clone MEM-09
Isotype IgG1
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity Chromatography on Protein A
Alternative Name Immunoglobulin
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Research Area Secondary Antibodies, Virology
Application Notes Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (paraffin sections). Immunocytochemistry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Horejsí, Hilgert, Kristofová, Bazil, Bukovský, Kulhánková et al.: "Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte ..." in: Folia biologica, Vol. 32, Issue 1, pp. 12-25, 1986 (PubMed).