LAT2 antibody (AA 196-212)

Catalog No. ABIN120728

The Mouse Monoclonal anti-LAT2 antibody is suitable to detect LAT2 in samples from Human. It has been validated for WB, IP and IHC (p).

Quick Overview for LAT2 antibody (AA 196-212) (ABIN120728)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NAP-03

Product Details anti-LAT2 Antibody

Binding Specificity: AA 196-212

Specificity: The antibody recognizes the defined epitope (aa 196-212) of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide corresponding to aa 196-212 of human NTAL coupled to KLH

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot: Recommended dilution: 1-2 mg/mL, incubation 60 min. Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: LAT2 / WBSCR15

Background: NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein

Gene ID: 7462

UniProt: Q9GZY6

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling