MAP2 antibody

Catalog No. ABIN120770

The Mouse Monoclonal anti-MAP2 antibody is suitable to detect MAP2 in samples from Human, Mouse and Pig. It has been validated for WB, IP, IF, IHC (p), IHC (fro) and EIA.

Quick Overview for MAP2 antibody (ABIN120770)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: MT-08

Product Details anti-MAP2 Antibody

Specificity: The antibody recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).

Purification: Protein A Chromatography

Purity: > 95 % (by SDS-PAGE).

Immunogen: Microtubule protein (bovine brain) enriched for kinesin

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections (use mouse brain as positive tissue). Immunocytochemistry (use human neuroblastoma SH-SY5Y as positive control). Western blot (use porcine brain or MAP2 as positive control). Immunoprecipitation (use porcine brain as positive material). ELISA (use porcine brain or MAP2 as positive control).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 15 mM Sodium Azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP-2

Background: MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gene ID: 4133

UniProt: P11137