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CD3 antibody

CD3 Reactivity: Chicken FACS, IP, IHC (fro) Host: Mouse Monoclonal CT-3 unconjugated

Catalog No. ABIN121123

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
181

90

22

15

15

10

8

8

7

5

3

3

2

1
Chicken
Host
206

45

14

13

9
Mouse
Compatible Secondaries
Clonality
276

7
Monoclonal
Conjugate
85

32

27

22

21

11

7

6

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is un-conjugated
Application
259

79

53

44

42

35

28

23

9

8

7

7

4

3

2

2

2

2

2

2

2

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification

Ion Exchange Chromatography

Immunogen

Chicken thymocytes and Ig -ve peripheral blood mononuclear cells. Lymph node cells from immunised BALB/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line

Clone

CT-3

Isotype

IgG1

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CD3 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, Func, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

Flow Cytometry: 1:50, Use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5 mg/mL

Buffer

Borate buffered saline, None, None

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date

12 months
Target

CD3

Alternative Name

CD3 (CD3 Products)

Synonyms

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background

In the chicken, CD3 is expressed by approximately 30 % of thymocytes, 70 % of peripheral blood mononuclear cells, but less than 1 % of cells in the bursa and bone marrow.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway