TNF alpha antibody

Catalog No. ABIN121800

The Rabbit Polyclonal anti-TNF alpha antibody (ABIN121800) specifically detects TNF alpha in WB and EIA. The antibody is reactive with Cow samples.

Quick Overview for TNF alpha antibody (ABIN121800)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TNF alpha Antibody

Specificity: This antibody is specific for tumour necrosis factor alpha (TNF alpha).

Purification: Affinity chromatography on protein G

Immunogen: Recombinant bovine TNF alpha

Application Details

Application Notes: ELISA: 1 μg/mL - 10 μg/mL. Western Blot: 1 μg/mL - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Preservative: Azide free

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 280943

UniProt: Q06599

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome