TNF alpha antibody

Catalog No. ABIN121848

The Rabbit Polyclonal anti-TNF alpha antibody (ABIN121848) specifically detects TNF alpha in WB and EIA. The antibody is reactive with Horse samples.

Quick Overview for TNF alpha antibody (ABIN121848)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TNF alpha Antibody

Specificity: This antibody is specific for tumour necrosis factor alpha (TNF alpha). This antibody recognises both native and recombinant TNF alpha.

Purification: Affinity chromatography on protein G

Immunogen: Recombinant equine TNF alpha

Application Details

Application Notes: ELISA: 1 μg/mL - 10 μg/mL. Western Blot: 0.1 μg/mL - 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Preservative: Azide free

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: TNF alpha is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 100033834

UniProt: P29553

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome