BIM antibody (N-Term)

Catalog No. ABIN121852

This Rabbit Polyclonal antibody specifically detects BIM in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BIM antibody (N-Term) (ABIN121852)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BIM Antibody

Binding Specificity: AA 22-40, N-Term

Specificity: This antibody is specific for Bcl-2 interacting mediator of cell death (Bim), also known as Bod.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 22 to 40 of human Bim

Isotype: IgG

Application Details

Application Notes: Western blot: 1:1000, detects a band of approximately 22 kDa in K562 and A549 whole cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Background: Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway. Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w. Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex. Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

Gene ID: 10018

UniProt: O43521

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity