Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1) (C-Term) antibody

Antigen: Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1)

Synonyms: ICE, P45, IL1BC, Il1bc, Ice, DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370, CASP1, LOC100033888

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121891

Additional Information

Alternative name: Caspase-1

UniProt: P29466

Immunogen: A 16 amino acid peptide located near human Caspase-1 carboxy terminus

Cross-Reactivity: Human

Description: Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases. Alternate Names: CASP1, ICE, p45, IL-1BC, IL1BC, IL1BCE, Caspase 1, Caspase1, CASP 1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1 beta-converting enzyme.

Application Details

Application Notes: Western Blot: 0.5ug - 1.0ug/ml. Immunohistochemistry on paraffin sections: 0.5ug - 1.0ug/ml, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Recommended Positive Control: Human heart tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% Sodium Azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Kostura, Tocci, Limjuco et al.: "Identification of a monocyte specific pre-interleukin 1 beta convertase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 14, pp. 5227-31, 1989 (PubMed).

Miura, Zhu, Rotello et al.: "Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3." in: Cell, Vol. 75, Issue 4, pp. 653-60, 1993 (PubMed).

Ghayur, Banerjee, Hugunin et al.: "Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production." in: Nature, Vol. 386, Issue 6625, pp. 619-23, 1997 (PubMed).

Fantuzzi, Puren, Harding et al.: "Interleukin-18 regulation of interferon gamma production and cell proliferation as shown in interleukin-1beta-converting enzyme (caspase-1)-deficient mice." in: Blood, Vol. 91, Issue 6, pp. 2118-25, 1998 (PubMed).