Caspase 1 antibody (C-Term)

Catalog No. ABIN121891

This Rabbit Polyclonal antibody specifically detects Caspase 1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Caspase 1 antibody (C-Term) (ABIN121891)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 1 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognises an epitope within the C-terminal region (CT) of Caspase-1, otherwise known as IL-1Beta converting enzyme. This antibody detects a cleaved subunit band of approximately 21 kDa in heart cell lysates (predicted precursor MWT 45.2 kDa).

Purification: Affinity chromatography

Immunogen: A 16 amino acid peptide located near human Caspase-1 carboxy terminus

Application Details

Application Notes: Western Blot: 0.5 μg - 1.0 μg/mL. Immunohistochemistry on paraffin sections: 0.5 μg - 1.0 μg/mL, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Recommended Positive Control: Human heart tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background: Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome