Immunohistochemistry with formalin fixed paraffin embedded tissues: 20 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.7, containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
NR1F3 antibody, RORG antibody, RZR-GAMMA antibody, RZRG antibody, TOR antibody, Nr1f3 antibody, RORgamma antibody, Thor antibody, RAR related orphan receptor C antibody, RAR-related orphan receptor C antibody, RAR-related orphan receptor gamma antibody, RORC antibody, Rorc antibody
Background
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma t, a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.Synonyms: NR1F3, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, RORG, RZRG, Retinoid-related orphan receptor-gamma