CD46 antibody
| Antigen |
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| Clonality |
Monoclonal (MEM-258) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
Flow Cytometry (FACS) (44), Western Blotting (WB) (32), Immunoprecipitation (IP) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Functional Studies (Func) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), ELISA (1), ELISA (Detection) (1), Fluorescence Microscopy (FM) (1), Immunocytochemistry (ICC) (1)
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| Catalog no. |
ABIN125715 |
| Quantity |
0.1 mg (1 mg/ml) (Variants) |
| Price |
273.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Immunogen
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HPB-ALL human T cell line
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Format
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Azide Free
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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MEM-258
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Description
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CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembraneprotein that binds and inactivates C3b and C4b complement fragments, regulates Tcell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing(CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor forseveral human pathogens (both bacteria and viruses), and its ligation alteres T lymphocytepolarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function.CD46 is a protector of placental tissue and is also expressed on the inner acrosomalmembrane of spermatozoa.
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Specificity
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The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR)domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembraneprotein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelialcells, epithelial cells and fibroblast, it is negative on erythrocytes.
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Application Notes
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Western Blotting. Application note: Non-reducing conditions.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 ยต m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Immunology, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD46", type "Antibodies"
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Hosts
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Mouse (53), Rabbit (2)
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Reactivities
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Human (49), Cow (Bovine) (14), Pig (Porcine) (5)
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Applications
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Flow Cytometry (FACS) (44), Western Blotting (WB) (32), Immunoprecipitation (IP) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Functional Studies (Func) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), ELISA (1), ELISA (Detection) (1), Fluorescence Microscopy (FM) (1), Immunocytochemistry (ICC) (1)
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Conjugates
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FITC (10), PE (5), RPE (3), APC (2), Biotin (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Pacific Blue (1)
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