TRIM antibody (Intracellular) (PE)
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- Target See all TRIM (TRAT1) Antibodies
- TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))
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Binding Specificity
- AA 29-186, Intracellular
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TRIM antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
- No Cross-Reactivity
- Mouse
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Recombinant intracellular domain (aa 29-186) of human TRIM.
- Clone
- TRIM-04
- Isotype
- IgG2a
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- Application Notes
- Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
- Comment
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." in: The Journal of experimental medicine, Vol. 193, Issue 11, pp. 1269-84, (2001) (PubMed).
: "
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The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." in: The Journal of experimental medicine, Vol. 193, Issue 11, pp. 1269-84, (2001) (PubMed).
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- Target
- TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))
- Alternative Name
- TRIM (TRAT1 Products)
- Synonyms
- TCRIM antibody, TRIM antibody, C030046M14Rik antibody, Tcrim antibody, Trim antibody, T cell receptor associated transmembrane adaptor 1 antibody, T-cell receptor associated transmembrane adaptor 1 antibody, TRAT1 antibody, Trat1 antibody
- Background
- T cell receptor associated transmembrane adaptor 1,TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.,TRAT, TCRIM
- Gene ID
- 50852
- UniProt
- Q6PIZ9
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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