V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (C-Term) antibody

Antigen: V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyms: ERBB3

Epitope: C-Term

Clonality: Monoclonal (11A4)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN126793

Additional Information

Characteristics: Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

Alternative name: ERBB3 / HER3 (C-term)

Swiss-Prot: P21860

Immunogen: Synthetic petide conjugated to KLH.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 11A4

Description: ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsickinase activity due to an amino acid substitution within the ATP binding site. Uponheterodimerization with the other erbB family members, erbB3 becomes phosphorylated atmultiple tyrosine residues that represent docking sites for downstream signaling proteins,e. g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.

Specificity: This antibody specifically recognizes the C-terminus of erbB3 at 185 kDa. Species: Human. Others not tested.

Application Details

Application Notes: Western Blot: 0.5 ug/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 1 ml PBS / 0.09 % Na-azide / PEG and SucroseReconstitution: Restore with 1 ml H2O (15 min, RT).

Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Detection of endogenous erbB3 Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab erbB3-11A4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231