IGF1R antibody (C-Term)

Catalog No. ABIN126815

The Mouse Monoclonal anti-IGF1R antibody is suitable to detect IGF1R in samples from Human. It has been validated for WB.

Quick Overview for IGF1R antibody (C-Term) (ABIN126815)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7G11

Product Details anti-IGF1R Antibody

Binding Specificity: C-Term

Specificity: This antibody specifically recognizes the C-terminus of IGF1 receptor.

Characteristics: Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive control: Cell lysate from untreated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: CD221 / IGF1R

Background: The IGF1 receptor (IGF1R) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The IGF1 receptor is activated upon binding of the peptide hormones IGF1 and 2, leading to autophosphorylation of tyrosine residue 1131, 1135, and 1136 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residue 950 and 1316 regulate the assembly of signal transduction complexes.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

Molecular Weight: 97 kDa

Gene ID: 3480

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy