ERK1 antibody (pThr-Glu-pTyr) (FITC)

Catalog No. ABIN126830

The Mouse Monoclonal anti-ERK1 antibody is suitable to detect ERK1 in samples from Human, Mouse, Rat and Dog. It has been validated for IF.

Quick Overview for ERK1 antibody (pThr-Glu-pTyr) (FITC) (ABIN126830)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is conjugated to FITC

Application: Immunofluorescence (IF)

Clone: 12D4

Product Details anti-ERK1 Antibody (FITC)

Binding Specificity: pThr-Glu-pTyr

Specificity: This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). T he antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.

Characteristics: Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3

Purification: Subsequent thiophilic adsorption and size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH. Epitope: . . . pT-E-pY. . . Remarks: Mol. Weight: 42-44 kDa

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: 1 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Aliquote and freeze in liquid nitrogen. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Protect from light.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK

Gene ID: 5595

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins