PIN1 antibody

Catalog No. ABIN126850

Product Details anti-PIN1 Antibody

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIN1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes Human PIN1 in cell extracts at 16 kDa.

Characteristics: Synonyms: PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-transisomerase Pin1

Purification: Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Recombinant human PIN1

Clone: 8C10

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: PIN1 (PIN1 Products)

Synonyms: DOD antibody, UBL5 antibody, dod antibody, ubl5 antibody, pin1 antibody, pin1a antibody, 0610025L01Rik antibody, D9Bwg1161e antibody, pin1b antibody, zgc:73206 antibody, PPIase pin1 antibody, MdPin1 antibody, Pin1 antibody, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 antibody, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog antibody, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 antibody, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog antibody, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody, peptidyl-prolyl cis-trans isomerase Pin1 antibody, PIN1 antibody, Pin1 antibody, pin1 antibody, pin1.S antibody, pin1.L antibody, ppiD antibody, PITG_11123 antibody, LOC103419188 antibody

Background: PIN1 is a peptidyl-prolyl-cis-trans-isomerase (PPIase) that specifically interacts with serine phosphate-proline or threonine phosphate-proline motifs. Upon binding, PIN1 isomerizes the peptide bond form cis to trans. Known substrates of PIN1 are serveral mitotic phosphoproteins (e.g. cdc25) as well as phosphorylated p53, phosphorylated β-catenin and phosphorylated tau protein. It is assumed that the isomerization of phosphoproteins regulates their biological function.Synonyms: PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1

Gene ID: 5300

UniProt: Q13526