SHC1 antibody (N-Term)

Catalog No. ABIN126890

The Mouse Monoclonal anti-SHC1 antibody is suitable to detect SHC1 in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for SHC1 antibody (N-Term) (ABIN126890)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2-40E-005

Product Details anti-SHC1 Antibody

Binding Specificity: N-Term

Specificity: This antibody specifically recognizes the N- terminus of Shc/p66.

Characteristics: Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated Neuro 2A cells (See included Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Included Positive Control: Cell lysate from untreated Neuro 2A cells

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium-Azide, PEG and Sucrose, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: SHC1 / SHC

Background: Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1

Molecular Weight: 66 kDa

Gene ID: 6464

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2