SHC1 antibody (AA 272-559)

Catalog No. ABIN7435826

This anti-SHC1 antibody is a Rabbit Polyclonal antibody detecting SHC1 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for SHC1 antibody (AA 272-559) (ABIN7435826)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SHC1 Antibody

Binding Specificity: AA 272-559

Purpose: Polyclonal Antibody to SHC-Transforming Protein 1 (SHC1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SHC1. It has been selected for its ability to recognize SHC1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SHC-Transforming Protein 1 (SHC1) corresdonding to Glu272~His559 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:230-940Immunohistochemistry: 5-20 μg/mL,1:23-94Immunocytochemistry: 5-20 μg/mL,1:23-94Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: SHC1

Background: SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2