Cathepsin B antibody

Catalog No. ABIN127046

Product Details anti-Cathepsin B Antibody

Target: Cathepsin B (CTSB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of Cathepsin B.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 322 of human cathepsins B

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Alternative Name: Cathepsin B (CTSB Products)

Synonyms: APPS antibody, CPSB antibody, CB antibody, apps antibody, cpsb antibody, LOC692390 antibody, CTSB antibody, cathepsin B antibody, cathepsin B S homeolog antibody, CTSB antibody, Ctsb antibody, ctsb.S antibody, LOC692390 antibody

Background: Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.Synonyms: APP secretase, APPS, CPSB, CTSB, Cathepsin B1

Gene ID: 1508

NCBI Accession: NP_001899

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades