NOXA antibody

Catalog No. ABIN127296

The Rabbit Polyclonal anti-NOXA antibody (ABIN127296) specifically detects NOXA in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NOXA antibody (ABIN127296)

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOXA Antibody

Specificity: The antibody recognizes mainly ~16 kDa band. A ~34 kDa band is also detected to the lesser extent.

Purification: Protein A chromatography

Immunogen: Synthetic peptide surrounding amino acid 100 of human Noxa

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: BPS containing 0.5 % BSA, 30 % glycerol, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Target Details for NOXA

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Alternative Name: NOXA

Background: Noxa is a BH3-only member of the Bcl-2 family. Expression of Noxa gene involves direct activation of its promotor by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as Bcl-xL and Mcl-1. Blocking the endogenous Noxa induction results in the suppression of apoptosis. These suggest Noxa may represent a candidate mediator of p53-induced apoptosis.Synonyms: PMA-induced protein 1, PMAIP1, Phorbol-12-myristate-13-acetate-induced protein 1

Gene ID: 5366

NCBI Accession: NP_066950

UniProt: Q13794

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus