Chicken, Sheep (Ovine), Dog (Canine), Rat (Rattus), Chimpanzee
Characteristics
Concentration Definition: by UV absorbance at 280 nm
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein (see below).
ASAP1
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract. This antibody recognizes both phosphorylated and non-phosphorylated ASAP1 at amino acid Y782.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.95 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Sato, Zhao, Imai, Simister, Feller, Trackman, Kirsch, Sonenshein: "Inhibition of CIN85-mediated invasion by a novel SH3 domain binding motif in the lysyl oxidase propeptide." in: PLoS ONE, Vol. 8, Issue 10, pp. e77288, (2013) (PubMed).
Target
ASAP1
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))
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Background
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein. Synonyms: Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody