Mouse anti-Fish Ig (Heavy Chain) Antibody (FITC)

Catalog No. ABIN134268

Product Details

Target: Ig

Binding Specificity: Heavy Chain

Reactivity: Fish, Rainbow Trout

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Specificity: Immunoglobin heavy chain in Rainbow trout species

Characteristics: The FITC anti-Rainbow trout Ig monoclonal antibody can be used to directly detect a generalized or pathogen-specific increase in immunoglobulin level. This monoclonal antibody only recognizes the immunoglobulin heavy chain found in Oncorhynchus species (ie. Coho, Chum, Sockeye and Chinook) but not the Atlantic salmon heavy chain. This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-Rainbow Trout Ig monoclonal antibody. See ELISA protocol below. Specific uses for this monoclonal antibody include ELISA, Western blots and immunofluorescence (ie. FACS).

Purification: Immunoaffinity chromatography

Immunogen: Purified Rainbow trout Ig (heavy and light chains) . Fusion Partner: X63Ag8.653 myeloma cells

Isotype: IgG1

Application Details

Application Notes: This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-Rainbow Trout Ig monoclonal antibody. See ELISA protocol below. Specific uses for this monoclonal antibody include ELISA, Western blots and immunofluorescence (ie. FACS).

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Handling Advice: Product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C. For long term storage aliquot and freeze unused portion at -20C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Target Details

Target: Ig

Abstract: Ig Products