IL-33 Protein (AA 112-270) (His tag)
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- Target See all IL-33 (IL33) Proteins
- IL-33 (IL33) (Interleukin 33 (IL33))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 112-270
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL-33 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Specificity
- Binds to human ST2.
- Cross-Reactivity
- Human
- Characteristics
- Human IL-33 (aa 112-270) is fused at the C-terminus to a His-tag.
- Purity
- >90 % (SDS-PAGE)
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- <1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product IL33 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activates human ST2-dependent NF-kappaB pathway.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C. - Expiry Date
- 3 months
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Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 88, Issue 11, pp. 1245-53, (2008) (PubMed).
: "An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 9, pp. 5981-9, (2008) (PubMed).
: "IL-33 can promote survival, adhesion and cytokine production in human mast cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 10, pp. 971-8, (2007) (PubMed).
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Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 88, Issue 11, pp. 1245-53, (2008) (PubMed).
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- Target
- IL-33 (IL33) (Interleukin 33 (IL33))
- Alternative Name
- IL-33 (IL33 Products)
- Synonyms
- IL33 Protein, C9orf26 Protein, DVS27 Protein, IL1F11 Protein, NF-HEV Protein, NFEHEV Protein, RP11-575C20.2 Protein, 9230117N10Rik Protein, Il-33 Protein, Il1f11 Protein, RGD1311155 Protein, interleukin 33 Protein, IL33 Protein, Il33 Protein
- Background
- Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30 kDa human IL33 is converted by CASP1 to a 18 kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
- Molecular Weight
- ~18kDa (SDS-PAGE)
- UniProt
- Q2YEJ5
- Pathways
- Production of Molecular Mediator of Immune Response
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