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Collagen, Type I (COL1) antibody (Biotin)

Antigen

Collagen, Type I (COL1)
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Biotin
Application
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
Catalog no. ABIN135047
Quantity 0,2 mg  (Variants)
Price 420.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Variants back to top

Alternative name Type I Collagen
Description Reacts with conformational determinants on human type I collagen as demonstrated by ELISA. May react with type I collagen from other species. Exhibits <10% cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Application Details
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Application Notes WORKING DILUTIONS Immunocytochemistry/ Immunohistochemistry: Purified (1:10-1:20), Biotin (1:20-1:40). ELISA: Biotin (1:1000-1:4000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. *Please note that harsh chemicals or procedures involving heat may denature collagen epitopes needed for proper binding. We recommend avoiding such applications when using this antibody.
Storage The purified (UNLB) antibody is supplied as 0.2 mg of purified immunoglobulin in 0.5 mL (C = 0.4 mug/mL) of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.2 mg in 0.5 mL (C = 0.4 mug/mL) of PBS/NaN3. Store at 2-8C. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "Collagen, Type I (COL1)", type "Antibodies"
Hosts Rabbit (71), Goat (16), Mouse (16), Bovine (2), Rat (2), Human (1)
Reactivities Human (70), Rat (Rattus) (30), Mouse (Murine) (27), Cow (Bovine) (25), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (16), Rabbit (15), Chicken (8), Fish (3), Amphibian (2), Bird (Avian) (2), Mammalian (2), Pig (Porcine) (2)
Applications ELISA (77), Immunohistochemistry (Frozen Sections) (IHC (fro)) (57), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Western Blotting (WB) (24), Flow Cytometry (FACS) (18), Radioimmunoassay (RIA) (18), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Dot Blot (Dot) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Solid Phase Radioimmunoassay (SP-RIA) (1)
Conjugates Biotin (10), None (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)