Collagen Type I antibody (AA 1321-1400)

Catalog No. ABIN670386

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Binding Specificity: AA 1321-1400

Reactivity: Human, Rat, Mouse, Rabbit, Dog, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Specificity: This antibody is produced using a peptide that is found in the C-terminal propeptide region.

Cross-Reactivity: Dog, Fish, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Collagen Type I antibody (ABIN670386)

Yang, Shi, Wu, Li, Zhang, Li, Zhai, Jia, Dang: "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." in: World journal of gastroenterology, Vol. 23, Issue 7, pp. 1203-1214, (2017) (PubMed).

Tian, Li, Yang, Luo, Dai, Liu, Song, Zhang: "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." in: Experimental and therapeutic medicine, Vol. 13, Issue 3, pp. 877-884, (2017) (PubMed).

Yu, Liu, Sun, Wang, Wang, Zhao, Dai, Zhao, Zhang, Li, Han, Lu, Dong, Liu, Yu, Li: "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." in: Oncotarget, Vol. 8, Issue 23, pp. 37705-37716, (2017) (PubMed).

Yang, Zhao, Wang, Liu, Ma, Huang, Liu: "Isolation and biological characterization of tendon-derived stem cells from fetal bovine." in: In vitro cellular & developmental biology. Animal, Vol. 52, Issue 8, pp. 846-56, (2017) (PubMed).

Long, Luo, Wang, Bates, Shetty: "Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells." in: Aging and disease, Vol. 8, Issue 3, pp. 301-313, (2017) (PubMed).

Çakir-Özkan, Bereket, Sener, Alici, Kabak, Önger: "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." in: The Journal of craniofacial surgery, Vol. 27, Issue 3, pp. 615-20, (2016) (PubMed).

Wang, Yang, Lin, Liu, Yang, Kuo, Chen: "Expandable Scaffold Improves Integration of Tissue-Engineered Cartilage: An In Vivo Study in a Rabbit Model." in: Tissue engineering. Part A, Vol. 22, Issue 11-12, pp. 873-84, (2016) (PubMed).

Cecen, Kozaci, Yuksel, Ustun, Ergur, Havitcioglu: "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." in: Materials science & engineering. C, Materials for biological applications, Vol. 69, pp. 437-46, (2016) (PubMed).

Wang, Li, Huang, Wang: "The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring." in: BioMed research international, Vol. 2014, pp. 419805, (2015) (PubMed).

Zhou, Wang, Song, He, He, He: "Antihypertensive and vascular remodelling effects of the imperatorin derivative OW1 in renovascular hypertension rats." in: Clinical and experimental pharmacology & physiology, Vol. 41, Issue 8, pp. 571-8, (2014) (PubMed).

McNary, Athanasiou, Reddi: "Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton." in: Tissue engineering. Part A, Vol. 20, Issue 5-6, pp. 921-9, (2014) (PubMed).

Luo, Zhang, Wang, Hu, Song, Su, Zhang: "Alendronate retards the progression of lumbar intervertebral disc degeneration in ovariectomized rats." in: Bone, Vol. 55, Issue 2, pp. 439-48, (2013) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Zhang, Ma, Xi, Zhang, Li: "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." in: BMC surgery, Vol. 11, pp. 5, (2011) (PubMed).

Xu, Chen, Huang, Varghese, Moorhead, Yan, Powis, Li, Ruan: "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 4, pp. F713-22, (2011) (PubMed).

Yang, Yang, Tu, Zheng, Cai, Wang: "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 6, pp. 981-91, (2011) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen 1 (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background:

Synonyms: Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1, Pro-collagen 1.

Background: This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Type I collagen levels have been linked to bone growth and formation. Levels of the C-terminal propeptide are indicative of collagen production in vivo. Elevated levels of C-terminal propeptide have been shown in diseases associated with high levels of bone turnover, including Pagets disease, hyperthyroidism, primary hyperparathyroidism and renal osteodystrophy. Slightly elevated levels of C-terminal propeptide have also been documented in some women in early menopause.

Molecular Weight: 161kDa

Gene ID: 1277