Collagen Type I antibody

Catalog No. ABIN135046

This Goat Polyclonal antibody specifically detects Collagen Type I in WB, ELISA, IHC (fro), IHC (p), IP, ICC, FACS and IEM. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep, Chicken, Pig, Hamster, Cat, Guinea Pig, Elephant, Chinchilla chinchilla and Tree shrew. It has been mentioned in 69+ publications

Quick Overview for Collagen Type I antibody (ABIN135046)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep, Chicken, Pig, Hamster, Cat, Guinea Pig, Elephant, Chinchilla chinchilla, Tree shrew

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoelectron Microscopy (IEM)

Product Details anti-Collagen Type I Antibody

Purpose: Goat Anti-Type I Collagen-UNLB

Specificity: Reacts with conformational determinants on type I collagen Referenced species reactivities include - Human 1-5,10,20,26,27,29,31-34 Chicken 17 Hamster 11,18 Mouse 24,27,28 Chinchilla 19 Porcine 5,21 Rat 5,13-15,23,25,27,35 Elephant 8 Rabbit 16,30 Bovine 6,20 Feline 9 Sheep 7 Canine 5,12 Guinea Pig 16 Tree Shrew 22

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Purified Anti-Type I Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, western blotting, and immunoprecipitation assays.

Purification: Purified by affinity chromatography on type I collagen covalently linked to agarose, cross-adsorbed against Collagen types II, III, IV, V, and VI for minimal reactivity

Immunogen: Type I collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type I collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Borate buffered saline, pH 8.2

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-Collagen Type I antibody (ABIN135046)

Corciulo, Castro, Coughlin, Jacob, Li, Fenyö, Rifkin, Kennedy, Cronstein: "Intraarticular injection of liposomal adenosine reduces cartilage damage in established murine and rat models of osteoarthritis." in: Scientific reports, Vol. 10, Issue 1, pp. 13477, (2020) (PubMed).

Bujor, El Adili, Parvez, Marden, Trojanowska: "Fli1 Downregulation in Scleroderma Myeloid Cells Has Profibrotic and Proinflammatory Effects." in: Frontiers in immunology, Vol. 11, pp. 800, (2020) (PubMed).

Becker, OConnell, Vo, Cain, Tampe, Bizarro, Sugimoto, McGow, Asara, Lovisa, McAndrews, Zielinski, Lorenzi, Zeisberg, Raza, LeBleu, Kalluri: "Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer." in: Cell reports, Vol. 31, Issue 9, pp. 107701, (2020) (PubMed).

Wietecha, Pensalfini, Cangkrama, Müller, Jin, Brinckmann, Mazza, Werner: "Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds." in: Nature communications, Vol. 11, Issue 1, pp. 2604, (2020) (PubMed).

Bigaeva, Puerta Cavanzo, Stribos, de Jong, Biel, Mutsaers, Jensen, Nørregaard, Leliveld, de Jong, Hillebrands, van Goor, Boersema, Bank, Olinga: "Predictive Value of Precision-Cut Kidney Slices as an Ex Vivo Screening Platform for Therapeutics in Human Renal Fibrosis." in: Pharmaceutics, Vol. 12, Issue 5, (2020) (PubMed).

Fried, Gilboa, Har-Zahav, Lavrut, Du, Karjoo, Russo, Shamir, Wells, Waisbourd-Zinman: "Extrahepatic cholangiocyte obstruction is mediated by decreased glutathione, Wnt and Notch signaling pathways in a toxic model of biliary atresia." in: Scientific reports, Vol. 10, Issue 1, pp. 7599, (2020) (PubMed).

Koopmans, Hesse, Nawijn, Kumawat, Menzen, Sophie T Bos, Smits, Bakker, van den Berge, Koppelman, Guryev, Gosens: "Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammation." in: Scientific reports, Vol. 10, Issue 1, pp. 6754, (2020) (PubMed).

Cui, Zhang, Leng, Blokzijl, Jansen, Dijkstra, Faber: "Pirfenidone Inhibits Cell Proliferation and Collagen I Production of Primary Human Intestinal Fibroblasts." in: Cells, Vol. 9, Issue 3, (2020) (PubMed).

Hreha, Collins, Daugherty, Twentyman, Paluri, Hunstad: "TGF��1 orchestrates renal fibrosis following Escherichia coli pyelonephritis." in: Physiological reports, Vol. 8, Issue 6, pp. e14401, (2020) (PubMed).

Wilson, Jarman, Mellin, Wilson, Waddell, Tsokkou, Younger, Raven, Bhalla, Noll, Olde Damink, Schaap, Chen, Bates, Banales, Dean, Henderson, Sansom, Kendall, Boulter: "Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors." in: Nature communications, Vol. 11, Issue 1, pp. 445, (2020) (PubMed).

Kawakubo, Tanaka, Ochi, Watanabe, Saka-Tanaka, Kanamori, Yoshioka, Yamashita, Goto, Itoh, Shirakawa, Kanai, Suzuki, Sawada, Ito, Ishigami, Fujishiro, Arima, Ogawa, Suganami: "Dipeptidyl peptidase-4 inhibition prevents nonalcoholic steatohepatitis-associated liver fibrosis and tumor development in mice independently of its anti-diabetic effects." in: Scientific reports, Vol. 10, Issue 1, pp. 983, (2020) (PubMed).

Ke, Yuan, Qin, Shi, Luo, Fang, Xu, Sun, Zen, Jiang, Zhou, Yang: "UCP2-induced hypoxia promotes lipid accumulation and tubulointerstitial fibrosis during ischemic kidney injury." in: Cell death & disease, Vol. 11, Issue 1, pp. 26, (2020) (PubMed).

Gasparitsch, Schieber, Schaubeck, Keller, Cattaruzza, Lange-Sperandio: "Tyrphostin AG490 reduces inflammation and fibrosis in neonatal obstructive nephropathy." in: PLoS ONE, Vol. 14, Issue 12, pp. e0226675, (2020) (PubMed).

Dobie, Wilson-Kanamori, Henderson, Smith, Matchett, Portman, Wallenborg, Picelli, Zagorska, Pendem, Hudson, Wu, Budas, Breckenridge, Harrison, Mole, Wigmore, Ramachandran, Ponting, Teichmann, Marioni et al.: "Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. ..." in: Cell reports, Vol. 29, Issue 7, pp. 1832-1847.e8, (2020) (PubMed).

Boland, Oró, Tølbøl, Thrane, Nielsen, Cohen, Tabor, Fernandes, Tovchigrechko, Veidal, Warrener, Sellman, Jelsing, Feigh, Vrang, Trevaskis, Hansen: "Towards a standard diet-induced and biopsy-confirmed mouse model of non-alcoholic steatohepatitis: Impact of dietary fat source." in: World journal of gastroenterology, Vol. 25, Issue 33, pp. 4904-4920, (2020) (PubMed).

Verhaart, Putker, van de Vijver, Tanganyika-de Winter, Pasteuning-Vuhman, Plomp, Aartsma-Rus, van Putten: "Cross-sectional study into age-related pathology of mouse models for limb girdle muscular dystrophy types 2D and 2F." in: PLoS ONE, Vol. 14, Issue 8, pp. e0220665, (2020) (PubMed).

Birket, Raibaud, Lettieri, Adamson, Letang, Cervello, Redon, Ret, Viale, Wang, Biton, Guillemot, Mikol, Leonard, Hanley, Orsini, Itier: "A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology." in: Stem cell reports, Vol. 13, Issue 2, pp. 380-393, (2020) (PubMed).

Ling, Li, Wang, Meng, Li, Zhang: "Carvedilol improves liver cirrhosis in rats by inhibiting hepatic stellate cell activation, proliferation, invasion and collagen synthesis." in: Molecular medicine reports, Vol. 20, Issue 2, pp. 1605-1612, (2020) (PubMed).

Del Campo, Sánchez-López, Salaices, von Kleeck, Expósito, González-Gómez, Cussó, Guzmán-Martínez, Ruiz-Cabello, Desco, Assoian, Briones, Andrés: "Vascular smooth muscle cell-specific progerin expression in a mouse model of Hutchinson-Gilford progeria syndrome promotes arterial stiffness: Therapeutic effect of dietary nitrite." in: Aging cell, Vol. 18, Issue 3, pp. e12936, (2020) (PubMed).

Marchetti, Burwell, Peterson, Cann, Hanna, Li, Ongstad, Boyd, Kennedy, Zhao, Rickert, Grimsby, DallAcqua, Wu, Tsui, Borrok, Gupta: "Targeted drug delivery via caveolae-associated protein PV1 improves lung fibrosis." in: Communications biology, Vol. 2, pp. 92, (2020) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: TypeI Collagen

Background:

Description: Goat Anti-Type I Collagen-UNLB

NCBI_Official_Symbol: COL1A1, COL1A2

Synoynms: COL1

Gene ID: 1277

UniProt: P02452