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Phosphotyrosine antibody (HRP)

Details for Product No. ABIN135060, Supplier: Log in to see
Antigen
Reactivity
Amino Acid
109
52
45
29
29
27
25
9
9
5
1
Host
Mouse
180
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
27
23
17
16
7
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
179
110
84
77
73
53
28
27
11
7
7
2
1
1
1
1
1
Supplier
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Clone PY20
Isotype IgG2b
Characteristics The antibody detects: Phosphorylated tyrosine and phenylphosphate
Purification Purified
Target Type Amino Acid
Background Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Working Dilution:
ELISA: 1:1,000-1:4,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Format Liquid
Buffer Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).