ITGA4 antibody

Catalog No. ABIN135079

This Rat Monoclonal antibody specifically detects ITGA4 in FACS, IP, IHC (fro), BR and Adh Act. It exhibits reactivity toward Human and Mouse.

Quick Overview for ITGA4 antibody (ABIN135079)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Adhesion Activation (Adh Act)

Clone: PS-2

Product Details anti-ITGA4 Antibody

Purpose: Rat Anti-Mouse CD49d-LE/AF

Specificity: Mouse/Human CD49d, Mr 150 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD49d antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, blocking, and adhesion assays.

Endotoxin Level: Low Endotoxin

Immunogen: P815 cells

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: CD49d, the 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150 kDa) VLA-4 belongs to the 1-integrin family, which functions both as cell-matrix and cell-cell receptors.1-3 As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive.

Gene ID: 16401

UniProt: Q00651

Pathways: Integrin Complex