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ITGAL antibody (Integrin alpha-L) (PE)

Details for Product anti-ITGAL Antibody No. ABIN135682, Supplier: Log in to see
Antigen
  • (p180)
  • CD11A
  • Cd11a
  • CD11a
  • LFA-1
  • LFA-1A
  • LFA1A
  • Ly-15
  • Ly-21
Reactivity
Human
480
237
47
22
11
11
8
8
4
3
Host
Mouse
498
210
77
17
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is conjugated to PE
101
89
58
19
18
16
15
14
13
13
13
12
12
11
11
11
11
9
9
8
4
3
3
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
682
229
167
142
108
102
53
50
36
17
12
8
6
6
4
3
3
2
2
1
1
1
1
1
Supplier
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Clone 38
Isotype IgG2a
Characteristics The antibody detects: Human CD11a (LFA-1)
Alternative Name CD11a/LFA-1a (ITGAL Antibody Abstract)
Background CD11a, also known as leukocyte function-associated antigen-1 (LFA-1), represents the 180 kDa integrin L subunit which combines with the CD18 2 integrin subunit to form the L2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a/LFA-1 mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation.
Application Notes Working Dilution:
FACS: 10 µL/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Blocking studies, cell adhesion, cytotoxicity

Restrictions For Research Use only
Buffer Product is supplied as 100 tests in 1.0 mL of PBS / NaN3 and a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Lub, van Kooyk, Figdor: "Ins and outs of LFA-1." in: Immunology today, Vol. 16, Issue 10, pp. 479-83, 1995 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Dransfield, Hogg: "Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits." in: The EMBO journal, Vol. 8, Issue 12, pp. 3759-65, 1990 (PubMed).