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Selectin E + CD62e antibody (Selectin E)

Details for Product anti-SELE Antibody No. ABIN135713, Supplier: Log in to see
Antigen
  • SELE
  • CD62E
  • ELAM
  • ELAM1
  • ESEL
  • LECAM2
  • E-selectin
  • Elam
Reactivity
Human
272
61
30
20
4
4
2
2
1
1
1
Host
Mouse
170
106
40
11
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Selectin E + CD62e antibody is un-conjugated
34
32
21
10
3
3
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
158
142
120
74
62
56
28
21
20
16
8
8
8
4
3
2
2
2
2
2
2
2
1
1
Supplier
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Clone 1-2B6
Isotype IgG1
Characteristics The antibody detects: Human CD62E/E-selectin
Purification Purified
Alternative Name CD62E/E-selectin (SELE Antibody Abstract)
Background CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Inhibition of activity

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Tedder, Steeber, Chen et al.: "The selectins: vascular adhesion molecules." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 10, pp. 866-73, 1995 (PubMed).

Gearing, Newman: "Circulating adhesion molecules in disease." in: Immunology today, Vol. 14, Issue 10, pp. 506-12, 1994 (PubMed).