LAMP2 antibody (Lysosomal-Associated Membrane Protein 2)

Details for Product anti-LAMP2 Antibody No. ABIN135832, Supplier: Log in to see
Antigen
  • cd107b
  • lamp-2
  • lampb
  • LAMP2
  • lamp2
  • DKFZp459J126
  • LAMP-2
  • LGP-B
  • CD107b
  • Lamp-2
  • Lamp-2a
  • Lamp-2b
  • Lamp-2c
  • Mac3
  • LAMPB
  • LGP110
  • lysosomal-associated membrane protein 2
  • Lysosome-associated membrane glycoprotein 2
  • lysosomal membrane glycoprotein B
  • LAMP2
  • lamp2
  • LOC100218047
  • LOC100222683
  • Lgpb
  • Lamp2
Alternatives
anti-Human LAMP2 antibody for Cytometry by Time of Flight
Reactivity
Human
296
198
44
43
18
9
3
2
Host
Mouse
169
144
104
2
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP2 antibody is un-conjugated
48
36
29
22
11
10
7
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
240
180
105
97
95
94
94
58
54
13
12
7
5
3
2
2
2
2
2
1
1
1
1
1
Options
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Clone H4B4
Isotype IgG1
Characteristics The antibody detects: Human CD107a/LAMP0
Purification Purified
Alternative Name CD107b/LAMP-2 (LAMP2 Antibody Abstract)
Background CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium.
Pathways Autophagy
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Product cited in: Fritsch, Stephan, Tchikov, Winoto-Morbach, Gubkina, Kabelitz, Schütze: "Cell Fate Decisions to TNF Regulated by K63 Ubiquitination of TNF-Receptor 1." in: Molecular and cellular biology, 2014 (PubMed).

Background publications Sawada, Lowe, Fukuda: "E-selectin-dependent adhesion efficiency of colonic carcinoma cells is increased by genetic manipulation of their cell surface lysosomal membrane glycoprotein-1 expression levels." in: The Journal of biological chemistry, Vol. 268, Issue 17, pp. 12675-81, 1993 (PubMed).

Sawada, Jardine, Fukuda: "The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes." in: The Journal of biological chemistry, Vol. 268, Issue 12, pp. 9014-22, 1993 (PubMed).

Febbraio, Silverstein: "Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein." in: The Journal of biological chemistry, Vol. 265, Issue 30, pp. 18531-7, 1990 (PubMed).

Chen, Cha, Yuksel, Gracy, August: "Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens." in: The Journal of biological chemistry, Vol. 263, Issue 18, pp. 8754-8, 1988 (PubMed).