NSF antibody (AA 151-250) (HRP)

Catalog No. ABIN1408082

Product Details anti-NSF Antibody (HRP)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Binding Specificity: AA 151-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NSF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF (NSF Products)

Synonyms: SKD2 antibody, AI316878 antibody, AU020090 antibody, AU067812 antibody, NSF antibody, 18.m06598 antibody, nsf antibody, si:bz18k17.1 antibody, wu:fj33g11 antibody, N-ethylmaleimide sensitive factor antibody, T1J1.4 antibody, T1J1_4 antibody, NEM-sensitive fusion protein antibody, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antibody, N-ethylmaleimide sensitive fusion protein antibody, N-ethylmaleimide-sensitive factor antibody, N-ethylmaleimide sensitive factor antibody, N-ethylmaleimide-sensitive fusion protein, putative antibody, N-ethylmaleimide sensitive factor L homeolog antibody, vesicle-fusing ATPase antibody, N-ethylmaleimide-sensitive factor a antibody, AAA-type ATPase family protein antibody, NSF antibody, Nsf antibody, nsf antibody, BBOV_II007200 antibody, TGME49_318510 antibody, nsf.L antibody, LOC100180685 antibody, LOC9310886 antibody, nsfa antibody

Background:

Synonyms: N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor like protein, N ethylmaleimide sensitive fusion protein, N ethylmaleimide sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, NEM sensitive fusion protein, NEM sensitive fusion protein, NEM-sensitive fusion protein, NSF, NSF_HUMAN, SKD 2, SKD2, SKD2, Vesicle fusing ATPase, Vesicle fusing ATPase, Vesicle-fusing ATPase, Vesicular fusion protein NSF, Vesicular fusion protein NSF, Vesicular-fusion protein NSF.

Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the -SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the -SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).