NHLH1 antibody (AA 51-133) (HRP)

Catalog No. ABIN1411892

Product Details anti-NHLH1 Antibody (HRP)

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Binding Specificity: AA 51-133

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHLH1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NHLH1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NHLH1

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Alternative Name: NHLH1 (NHLH1 Products)

Synonyms: hen1 antibody, nscl antibody, hen-1 antibody, nscl1 antibody, xhen1 antibody, bhlha35 antibody, NHLH1 antibody, HEN1 antibody, NSCL antibody, NSCL1 antibody, bHLHa35 antibody, Hen1 antibody, Nscl antibody, Tal2 antibody, nescient helix-loop-helix 1 S homeolog antibody, nescient helix-loop-helix 1 L homeolog antibody, nescient helix-loop-helix 1 antibody, nescient helix loop helix 1 antibody, nhlh1.S antibody, nhlh1.L antibody, NHLH1 antibody, nhlh1 antibody, Nhlh1 antibody

Background:

Synonyms: bHLHa35, Class A basic helix-loop-helix protein 35, Helix-loop-helix protein 1, HEN-1, HEN1, HEN1_HUMAN, Nescient helix loop helix 1, Nhlh1, NSCL, NSCL-1, NSCL1, OTTHUMP00000031860.

Background: The helix-loop-helix (HLH) structures are known motifs commonly found in membrane-active and DNA-binding proteins. The helix-loop-helix proteins HEN1 and HEN2 are DNA-binding proteins that may be involved in cell-type determination in the early nervous system. Studies of expression in normal tissues have demonstrated expression of NHLH1/NSCL-1 and NHLH2/NSCL-2, the genes encoding HEN1 and HEN2, in the developing central and peripheral nervous system, specifically in developing neurons.