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ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30)

Details for Product anti-ATG5 Antibody No. ABIN1449605, Supplier: Log in to see
Antigen
  • ATG5
  • CG1643
  • DmAtg5
  • Dmel\\CG1643
  • atg5
  • apg5l
  • zgc:100934
  • ATATG5
  • AUTOPHAGY 5
  • MKP11.20
  • MKP11_20
  • APG5
  • APG5-LIKE
  • APG5L
  • ASP
  • hAPG5
  • 2010107M05Rik
  • 3110067M24Rik
  • AW319544
  • Apg5l
  • Atg5l
  • C88337
  • Paddy
Epitope
AA 1-30, N-Term
47
18
14
13
13
12
11
8
7
7
6
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
241
81
60
14
11
10
7
6
5
3
2
2
2
1
1
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1
Host
Rabbit
185
42
14
11
2
Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
11
11
10
9
9
9
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
205
112
100
22
21
11
7
5
4
2
2
1
1
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of Human APG5L Genename: ATG5
Isotype Ig
Specificity This antibody recognizes Human and Mouse ATG5 (N-term). Other species not tested.
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name APG5L / ATG5 (ATG5 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
Molecular Weight 32447 Da
Gene ID 9474
NCBI Accession NP_004840
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Formalin-Fixed, Paraffin-Embedded human breast carcinoma tissue reacted with Autophag...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Formalin-Fixed, Paraffin-Embedded human cancer tissue reacted with ATG5 Antibody (N-t...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Western blot analysis of APG5L (arrow) using ATG5 Antibody (N-term) Cat.-No AP32173PU...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Western blot analysis of ATG5 Antibody (N-term) in Hela cell lysates, which were trea...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Western blot analysis using ATG5 Antibody (N-term) in Hela cell line lysates (35ug/la...
Western Blotting (WB) image for anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (AA 1-30) (ABIN1449605) Western blot analysis using ATG5 Antibody (N-term) Cat.-No AP32173PU-N to detect APG5...
Background publications Huang, Chegini, Chua et al.: "Macroautophagy in sporadic and the genetic form of Parkinson's disease with the A53T α-synuclein mutation." in: Translational neurodegeneration, Vol. 1, Issue 1, pp. 2, 2012 (PubMed).

Schober, Parlato, Huber et al.: "Cell loss and autophagy in the extra-adrenal chromaffin organ of Zuckerkandl are regulated by glucocorticoid signalling." in: Journal of neuroendocrinology, Vol. 25, Issue 1, pp. 34-47, 2013 (PubMed).

Keil, Höcker, Schuster et al.: "Phosphorylation of Atg5 by the Gadd45?-MEKK4-p38 pathway inhibits autophagy." in: Cell death and differentiation, Vol. 20, Issue 2, pp. 321-32, 2013 (PubMed).

Lépine, Allegood, Edmonds et al.: "Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switched to apoptosis by calpain-mediated autophagy-related gene 5 (Atg5) cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 52, pp. 44380-90, 2011 (PubMed).

Chiu, Ho, Wang: "Arsenic trioxide induces autophagy and apoptosis in human glioma cells in vitro and in vivo through downregulation of survivin." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 9, pp. 927-41, 2011 (PubMed).

Lépine, Allegood, Park et al.: "Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy." in: Cell death and differentiation, Vol. 18, Issue 2, pp. 350-61, 2011 (PubMed).

Ferreri, Illerhaus, Zucca et al.: "Flows and flaws in primary central nervous system lymphoma." in: Nature reviews. Clinical oncology, Vol. 7, Issue 8, pp. doi:10.1038/nrclinonc.2010.9-c1; author reply doi:10:1038/nrclinonc.2010.9-c2, 2010 (PubMed).

Xia, Zhang, Chen et al.: "Control of basal autophagy by calpain1 mediated cleavage of ATG5." in: Autophagy, Vol. 6, Issue 1, pp. 61-6, 2010 (PubMed).

Norberg, Gogvadze, Ott et al.: "An increase in intracellular Ca2+ is required for the activation of mitochondrial calpain to release AIF during cell death." in: Cell death and differentiation, Vol. 15, Issue 12, pp. 1857-64, 2008 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Mizushima, Yamamoto, Hatano et al.: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).

Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Hammond, Brunet, Johnson et al.: "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast." in: FEBS letters, Vol. 425, Issue 3, pp. 391-5, 1998 (PubMed).

Grand, Milner, Mustoe et al.: "A novel protein expressed in mammalian cells undergoing apoptosis." in: Experimental cell research, Vol. 218, Issue 2, pp. 439-51, 1995 (PubMed).