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ATG14 antibody (ATG14 Autophagy Related 14 Homolog (S. Cerevisiae)) (Middle Region)

Details for Product anti-ATG14 Antibody No. ABIN1449976, Supplier: Log in to see
Antigen
  • 4832427M01
  • ATG14L
  • Atg14L
  • BARKOR
  • D14Ertd114e
  • D14Ertd436e
  • KIAA0831
  • RGD1304610
Epitope
Middle Region
16
15
12
4
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
41
12
11
Host
Rabbit
41
Clonality
Polyclonal
Conjugate
This ATG14 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
38
22
19
5
3
2
2
1
Supplier
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Immunogen ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of Human ATG14
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name ATG14 / Barkor
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.Synonyms: Atg14L, Beclin 1-associated autophagy-related key regulator, KIAA0831
Molecular Weight 54 kDa
Gene ID 22863
NCBI Accession NP_055739
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-ATG14 antibody (ATG14 Autophagy Related 14 Homolog (S. Cerevisiae)) (Middle Region) (ABIN1449976) Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 Anti...
Background publications Matsunaga, Morita, Saitoh, Akira, Ktistakis, Izumi, Noda, Yoshimori: "Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 511-21, 2010

Itakura, Kishi, Inoue, Mizushima: "Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5360-72, 2008

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994