BAG4 antibody (AA 349-363)

Catalog No. ABIN1493862

Product Details anti-BAG4 Antibody

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Binding Specificity: AA 349-363

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BAG4 / SODD.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GNATSDHPNNQDQSS from an internal region of human BAG4 / SODD (NP_004865.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 60  kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6  kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun,23(11):4026-33., PMID: 12748303). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 20  kDa. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BAG4

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Alternative Name: BAG4 / SODD (BAG4 Products)

Synonyms: BAG-4 antibody, SODD antibody, BAG4 antibody, fd20a04 antibody, wu:fd20a04 antibody, si:dkey-220o5.3 antibody, 2410112I15Rik antibody, ATBAG4 antibody, BCL-2-associated athanogene 4 antibody, BCL2 associated athanogene 4 antibody, BCL2-associated athanogene 4 antibody, BCL-2-associated athanogene 4 antibody, BAG4 antibody, Bag4 antibody, bag4 antibody

Background: Name/Gene ID: BAG4
Family: Apoptosis

Synonyms: BAG4, BAG-4, Bcl-2-associated athanogene 4, BCL2-associated athanogene 4, Silencer of death domains, SODD

Gene ID: 9530

NCBI Accession: NP_004865

UniProt: O95429

Pathways: Regulation of Actin Filament Polymerization