RACGAP1 antibody (pSer387)

Catalog No. ABIN1531650

The Rabbit Polyclonal anti-RACGAP1 antibody (ABIN1531650) specifically detects RACGAP1 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for RACGAP1 antibody (pSer387) (ABIN1531650)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-RACGAP1 Antibody

Binding Specificity: AA 353-402, pSer387

Purpose: Rac GAP1 (phospho Ser387) Antibody

Specificity: Phospho-Rac GAP1 (S387) Polyclonal Antibody detects endogenous levels of Rac GAP1 protein only when phosphorylated at S387.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human GTPase Activating Protein around the phosphorylation site of Ser387. AA range:353-402

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: GTPase Activating Protein

Background:

Synonyms: RACGAP1, KIAA1478, MGCRACGAP, Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolg, CYK4, HsCYK-4

Background: This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016],

Gene Name: RACGAP1

Protein Name: Rac GTPase-activating protein 1

Molecular Weight: 71 kDa

Gene ID: 29127

OMIM: 604980

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor