Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC)

Catalog No. ABIN1536521

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Characteristics: Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is developed in goat using purified mouse IgG whole molecule. This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to FITC. Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is suitable for immunocytochemistry, immunohistochemistry and flow cytometry analysis as well as other antibody based fluorescent assays. Absorption Wavelength: 492 nm Emission Wavelength: 518 nmFluorochrome/Protein Ratio: 4. 2 moles FITC per mole of Goat IgG.

Purification: Immunoaffinity chromatography

Immunogen: Purified mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Flow cytometry: 1:200-1:500Immunocytochemistry: 1:200-1:500Immunohistochemistry: 1:100-1:300Other Applications: user-optimized

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with 1 mL of deionized water (or equivalent) to an antibody concentration of 1 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing cycles.

Storage: -20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-0 °C. For long term storage, aliquot and store at -20 °C or below.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody