Spartan antibody (Middle Region)
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- Target See all Spartan (C1orf124) products
- Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))
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Binding Specificity
- Middle Region
- Reactivity
- Human, Mouse, Rat, Pig, Rabbit, Cow, Dog, Guinea Pig, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Spartan antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- ATNREPSAHD YWWAEHQKTC GGTYIKIKEP ENYSKKGKGK AKLGKEPVLA
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 92%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against SPRTN. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of Human SPRTN
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 489 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))
- Alternative Name
- SPRTN (C1orf124 Products)
- Synonyms
- Spartan antibody, c1orf124 antibody, C1orf124 antibody, DDDL1880 antibody, DVC1 antibody, PRO4323 antibody, dJ876B10.3 antibody, C28H1orf124 antibody, SprT-like N-terminal domain antibody, sprtn antibody, SPRTN antibody
- Background
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SPRTN is a regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. SPRTN is recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. It facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. It is also required for localization of DNA polymerase eta (POLH) to sites of UV damage.
Alias Symbols: C1orf124, DDDL1880, PRO4323, Spartan, dJ876B10.3
Protein Interaction Partner: H2BFS, UBR5, FAM120A, STRAP, CKAP4, MAGED2, HIST2H2BF, HIST1H2BK, POLD4, ZC3HAV1, POLD3, DDX17, SYNCRIP, TRIM28, LRPPRC, HUWE1, HNRNPDL, AKAP12, RBM39, H1FX, HIST1H2BH, HIST1H2BM, HIST1H2BN, HIST1H2BL, HIST1H2BG, VIM, VDAC1, UBB, TUFM, DNAJC7, TMPO, STAU1
Protein Size: 489 - Molecular Weight
- 55 kDa
- Gene ID
- 83932
- NCBI Accession
- NM_032018, NP_114407
- UniProt
- Q9H040
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