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Activating Transcription Factor 2 (ATF2) (pThr69/51 + pThr71/53) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Epitope
Alternatives

pThr69/51 + pThr71/53
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN155094
Quantity 0.1 mg  (0.5 mg/ml)
Price 380.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, CyclicAMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2,cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-bindingprotein 2
Alternative name ATF2 pThr69/51 + pThr71/53
Swiss-Prot P15336
Description The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family ofleucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiateCRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 bystress-activated kinases is necessary for transcriptional activation. Myc also inducesphosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 alsofunctions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B andH4 in vitro.
Specificity The antibody detects phosphorylated ATF-2 (Thr69 and Thr71). Species: Human, Mouse, Rat. Others not tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western Blot: 1-2 μg/ml. Immunoprecipitation: 10 μg/ml. Immunohistochemistry: 20 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Buffer PBS, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide
Storage Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Cell Cycle, Signaling, Kinases/Phosphatases
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (142), Mouse (16), Goat (4), Sheep (3)
Reactivities Human (162), Mouse (Murine) (111), Rat (Rattus) (98), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1)
Applications Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (31), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
Conjugates Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pThr69/51 (6), Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pSer62/44 (5), pThr69 or 51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer112/94 (4), pThr71/53 (4), pThr73/55 (3), S44 (2), S94 (2), PSer340/322 (1), S322 (1), pSer44 (1), pSer472 (1), pSer94 (1), pThr51 (1), pThr71 (1), pThr79/51 (1)