HSP70 antibody (AA 80-130)

Catalog No. ABIN1574060

Product Details anti-HSP70 Antibody

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Binding Specificity: AA 80-130

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-HSP70 Polyclonal Antibody detects endogenous levels of human HSP70 protein. Sequence homology predicts that it will also react with mouse and rat HSP70 protein.

Cross-Reactivity (Details): Rabbit Anti-HSP70 Polyclonal Antibody detects endogenous levels of human HSP70 protein. Sequence homology predicts that it will also react with mouse and rat HSP70 protein.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) within AA 80-130 of human HSP70 protein .

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-HSP70 antibody (ABIN1574060)

Cuadrado, Fernández-Velasco, Boscá, de Las Heras: "Labdane diterpenes protect against anoxia/reperfusion injury in cardiomyocytes: involvement of AKT activation." in: Cell death & disease, Vol. 2, pp. e229, (2011) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 (HSP70 Products)

Synonyms: APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody

Background: HSP70 is a member of the chaperonin family. It stabilizes existing proteins against aggregation and mediates the folding of newly translated polypeptides in both cytosol and organelles. HSP70 participates in all of these processes through its ability to recognize nonnative conformations of other proteins. It binds extended peptide segments with a net hydrophobic character exposed during translation and membrane translocation or following stress-induced damage.Rabbit Anti-HSP70 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 80-130 of human HSP70 protein (Swiss Prot: P08107).