P35 (N-Term) antibody

Catalog No. ABIN1574113

Product Details

Target: P35

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: Rabbit Anti-p35 Polyclonal Antibody detects endogenous levels of mouse and rat p35. It is predicted to react with human p35 according to sequence homology.Positive Control: mouse brain, and rat brain.

Cross-Reactivity (Details): Rabbit Anti-p35 Polyclonal Antibody detects endogenous levels of mouse and rat p35. It is predicted to react with human p35 according to sequence homology.Positive Control: mouse brain, and rat brain.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from N-terminal of human p35 protein

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details

Target: P35

Synonyms: Anx1 antibody, p35 antibody, CDK5P35 antibody, CDK5R antibody, NCK5A antibody, p23 antibody, p25 antibody, p35nck5a antibody, 2010323F13Rik antibody, GFRP antibody, P35 antibody, annexin A1 antibody, cyclin dependent kinase 5 regulatory subunit 1 antibody, GTP cyclohydrolase I feedback regulator antibody, Anxa1 antibody, CDK5R1 antibody, Gchfr antibody

Target Type: Viral Protein

Background: Cyclin-dependent kinase 5 (Cdk5) is a member of the Cdk family of proteins that are involved in the regulation of the mitotic cell cycle. p35 protein is neuronal-specific regulatory subunit of Cdk5 and activates Cdk5 via its direct interaction. p35/Cdk5 activity is essential for neuronal morphogenesis and survival. p35 is cleaved by the Ca2+ dependent Cys protease calpain, which results in the generation of a C-terminal truncation product named p25. Containing all elements necessary for the activation of Cdk5, p25 activates Cdk5. Generation of p25 in the nervous system has been implicated in patients with Alzheimer-disease. It is suggested that p25/Cdk5 contributes to neuronal cell death after neurotoxic insults.Rabbit Anti-p35 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human p35 protein (Swiss Prot: Q15078).